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- Duke of Florence.
- Lord Cardinal, Brother to the Duke.
- Two Cardinals more.
- A Lord.
- Fabritio, Father to Isabella.
- Hippolito, Brother to Fabritio.
- Guardiano, Uncle to the Foolish Ward.
- The Ward, a rich yong Heir.
- Leantio, a Factor, Husband to Brancha.
- Sordido, the Wards Man.
- Livia, Sister to Fabritio.
- Isabella, Neece to Livia.
- Brancha, Leantio's Wife.
- Widow, his Mother.
- States of Florence.
- Citizens.
- A Prentice.
- Boys.
- Messenger.
- Servants.
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